Advanced microbiology practicals for MSc students

Hamari, Zsuzsanna and Pfeiffer, Ilona and Takó, Miklós and Vágvölgyi, Csaba: Advanced microbiology practicals for MSc students. (2019) [Note, textbook]

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English title

Advanced microbiology practicals for MSc students

Abstract

A tananyag áttekintést ad a környezeti és molekuláris mikrobiológiai technikákról; mikrobiális ökológiai módszerekről. A résztvevők elsajátítják a mikrobák manipulációjához szükséges alapvető technikákat és tanulmányozni fogják két fontos mikrobiális modellszervezet életciklusát. Ezen kívül megismerkednek a gombák által termelt extracelluláris enzimek kimutatásához szükséges módszerekkel.

English abstract

The aim of the subject: The courses are directed to students who have some skills in basic microbial techniques. It provides an overview of techniques generally used in environmental and molecular microbiology; methods used in microbial ecology are also presented. Participants will acquire techniques generally used to manipulate microorganisms and they will study the genetic background of the life cycle of two important eukariotic modell systems Aspergillus nidulans and Saccharomyces cerevisiae. Course description: - The sexual life cycle of Aspergillus nidulans (heterokaryon formation, diploid isolation, ascospore formation and genetic analysis of the progenies) - The parasexual life cycle in filamentous fungi (heterokaryon formation by anastomosis and protoplast fusion, diploid isolation, haploidization); - Study of the sexual life cycle of Saccharomyces cerevisiae (conjugation, diploid isolation, spore forming, random spore analysis); - The killer phenomenon in yeasts; - The presence of dsRNA viruses in Saccharomyces cerevisiae; - Counting of viable bacterial and fungal cells in soil samples; - Counting of psychro-, meso- and thermophilic bacteria in water samples; - Selective isolation of Pseudomonas and Bacillus strains from soil; - Selective isolation of Trichoderma strains from environmental samples; - In vitro testing of the microbial antagonism between bacteria and filamentous fungi; - Study of the extracellular lipase and esterase secretion of bacterial strains; - Detection and measurement of the cellulase, xylanase and phosphatase activity of Trichoderma strains; - Study of the extracellular amylase, chitinase and protease production of bacterial strains; - Isolation of laccase-producing filamentous fungi; - Determination of the copper tolerance of bacteria and fungi; - Determination of the susceptibility of Trichoderma strains to fungicides.

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