Macromolecule design and manipulation : practical textbook

Borsos Barbara Nikolett and Vedelek Balázs: Macromolecule design and manipulation : practical textbook. (2018) [Note, textbook]

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English title

Macromolecule design and manipulation : practical textbook

Abstract

This book is written to facilitate the understanding and implementation of the subject area thought in the Biology MSc practical course Macromolecule design and manipulation at the University of Szeged. We recommend this book to those students, who would like to extend their knowledge in particular by familiarising themselves with methods used in modern molecular biology and biochemistry. This book provides useful information on basic methods through a detailed coverage of experiments by expression and purification of a thermostable DNA polymerase, which will be performed by the participating students. Basic English language knowledge is required for the successful achievement of the course, which also gives the opportunity for the students to get familiar with some of the basic terminologies used in a molecular biology laboratory.

Item Type: Note, textbook
Contributors:
ContributionNameEmail
General editorBoros Imre Miklósborosi@bio.u-szeged.hu
English title: Macromolecule design and manipulation : practical textbook
Number of Pages: 304
Language: English
Pedagogic Type: note, book
End user role:
Training typeEducational type
BSc/BAUNSPECIFIED
MSc/MA/OMUNSPECIFIED
Task Purpose: They know the structure, function and regulation of biological systems; They know how to generate competent bacterial cells in the laboratory; They understand the concept of plasmid preparation; They know the theoretical background of agarose gelelectrophoresis and bacterial transformation; They are aware of the type of inducing agents used for heterologue protein expression; They are aware of the theoretical basis of sonication; They know the theoretical background of PCR and also the materials and equipment required to perform such an experiment; They know the differences between agarose- and polyacrylamide gel electrophoresis and the theoretical basis of them; They know the function of molecular weight markers; They understand the function of the protein purification process based on heat treatment; They know how to do the protein purification with salting out; They know how the dialysis works;
Projects: EFOP-3.4.3-16-2016-00014
Subproject: AP2
Subtopic: AP2_TTIK_2
Department: Biokémiai és Molekuláris Biológiai Tanszék, Biológiai Intézet
Faculty: Faculty of Science and Informatics
Additional Information: Illusztrációkkal
Uncontrolled English Keywords: pET expression system, lac operon, heterologous protein expression, Rosetta cell line, protein purification, PCR, salting out, dialysis, SDS-PAGE, transformation, DNA isolation, sonication
Subjects: 01. Natural sciences
01. Natural sciences > 01.06. Biological sciences
01. Natural sciences > 01.06. Biological sciences > 01.06.03. Biochemistry and molecular biology
01. Natural sciences > 01.06. Biological sciences > 01.06.03. Biochemistry and molecular biology > 01.06.03.04. Molecular biology
Date Deposited: 2018. Sep. 10. 11:53
Last Modified: 2018. Sep. 26. 10:52
URI: https://eta.bibl.u-szeged.hu/id/eprint/525

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